Production Of Chimeric Antibodies By Transgenic Chicken Bioreactors
Yoshinori Kawabe, Akitsu Hotta, Ken-ichiro Ono, Kazuhisa Esaka, Ken-ichi Nishijima, Masamichi Kamihira and Shinji Iijima Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan Abstract Recombinant antibodies have been successfully used as pharmaceuticals for diseases such as cancer and rheumatoid, and an effective mass production method for therapeutic antibodies is urgently desired. Transgenic chicken proposed as a...
Introduction Mjr
Glucose has been used as a sugar source in most cell culture media. However, it is not always suitable for the production of biologicals because it generates a high concentration of lactic acid by glycolysis, leading to a decrease in medium pH below optimal range for cell and product stability. Many investigators have shown that fructose was better than glucose in slow utilization and low lactate production Imamura et al., 1982 Petch et al., 1996 . Despite these facts, fructose has been little...
Introduction Bsz
Intracellular ROS cause irreversible damage to biological macromolecules, resulting in many diseases and cell transformation1. Electrolyzed reduced water ERW is a health beneficial alkaline drinking water which contains high concentration of dissolved hydrogen, and can scavenge intracellular ROS2-5. ERW was known to improve various diseases such as diabetes5,6, angiogenesis and cancer, and reduce hemodialysis-induced oxidative stress in end-stage renal disease patients7. We have revealed that...
Adcc
The ADCC assays of defucosylated IgG1 and S298A E333A K334A-IgG1 have been reported 1,2,4 . It was, however, unclear which modification led to greater enhancement of ADCC from the previous reports. In this study, we compared the ADCC activities of STD-IgG1, dFu-IgG1, and AAA-IgG1 to address the question. Two human B cell lymphoma cell lines expressing CD20 molecules, Raji and WIL-2S, were used as target cells. All IgG1 variants exhibited the same binding activity to these B cell lines in flow...
Observation Of Individual Cell Behaviors To Analyze Mitogenic Effects Of Sericin
Tomohiro Toyosawa1, Satoshi Terada1, Masahiro Sasaki2, Hideyuki Yamada2, and Masahiro Kino-oka3 1Department of Applied Chemistry and Biotechnology, University of Fukui 3-9-1 Bunkyo, Fukui 910-8507, Japan 2Technology Department, Seiren Co., Ltd. 1-10-1 Keya, Fukui 9188560, Japan 3Department of Chemical Science and Engineering, Osaka University 1-3 Machikaneyama-cho, Toyonaka 560-8531, Japan Abstract Mammalian cell cultures are used in many fields. Various biologically active agents are produced...
Trace element optimized prototypes for other cell lines
A trace element optimized transfected PER.C6 medium, PER.C6 CD Prototype, supported cell growth to a higher maximum viable cell density as well as increased cell culture longevity in both small-scale Figure 3 and large-scale data not shown agitated suspension than unsupplemented and supplemented AEM and the commercially available control. The CD Prototype also supported higher antibody production than the control Figure 3 . Two HeLa formulations with optimized trace elements have been...
The intracellular H2O2scavenging effect of fucoidan on HT1080 cells
Then, we checked to determine whether fucoidan could act as an H2O2 scavenger. DCFH-DA has been used as a substrate for measuring intracellular H2O2 production in HT1080 cells. DCFH-DA is hydrolyzed by esterases to dichlorofluorescin DCFH , which is trapped within the cell. This nonfluorescent molecule is then oxidized to fluorescent dichlorofluorescin DCF by action of cellular H2O2. HT1080 cells were pre-treated with MEM containing fucoidan for 24h, then incubated with 5 M DCFH-DA for 30 min...
References Uny
Brekke, O. H., and Sandlie, I., 2003, Therapeutic antibodies for human diseases at the dawn of the twenty-first century, Nat. Rev. Drug. Disc. 2 52-62. Bonnal, S., Boutonnet, C., Prado-Lourence, L., and Vanger, S., 2003, IRESdb the internal ribosome entry site database, Nucleic Acids Res. 31 427-428. Bosselmen, R. A., Hsu, R. V., Boggs, T., Hu, S., Brusxewski, J., Ou, S., Kozar, L., Martin, F., Green, C., Jacobson, F., Nicolson, M., Schultz, J. A., Seman, K. M., Rishell, W., and Stewart, R. G.,...
References Xui
1. Dhar, A., Young, M.R., and Colburn, N.H., Mol. Cell. Biochem. 234-235 185-193 2002 . 2. Shirahata, S., Kabayama, S., Nakano, M., Miura, T., Kusumoto, K., Gotoh, M., Hayashi H., Otsubo, K., Morisawa, S., and Katakura, Y., Biochem. Biphys. Res. Commun. 234 269-274 1997 . 3. Shirahata, S., Nishimura, T., Kabayama, S., Aki, D., Teruya, K., Otsubo, K., Morisawa, S., Ishii, Y., Gadek, Z., and Katakura, Y., Anti-Oxidative Water Improves Diabetes, in Animal Cell Technology From Target to Market,...
Neural stem cell cultivation
Neural stem cells NSCs were generated from E18 cortical tissue, processed and grown as neurospheres in non-agitated ultra low attachment ULA 6-well culture dish Corning in a 5 CO2, 37 C incubator 3-4 . The control medium was DMEM F12, 1x N2, 10 ng ml EGF and 20 ng ml basic FGF Invitrogen . The test medium was Neural sySTEM EX, a medium prototype, with 2 mM L-glutamine, 1x B27TM without retinol acetate RA , 10 ng ml EGF and 20 ng ml basic FGF. Viable cells were counted by trypan blue exclusion...
References Ept
Aotsuka, Y. and Naito, M., 1991, Enhancing effects of retinoic acid on monoclonal antibody production of human-human hybridomas, Cell. Immunol. 133 498-505. Ballow, M., Wang, X., Xiang, S. and Allen, C., 2003, Expression and regulation of nuclear retinoic acid receptors in human lymphoid cells, J. Clin. Immunol. 23 46-54. Desai, D., Michalak, M., Singh N. K. and Niles, R. M., 1996, Inhibition of retinoic acid receptor function and retinoic acid-regulated gene expression in mouse melanoma cells...
Springer
A C.I.P. Catalogue record for this book is available from the Library of Congress. ISBN-13 978-1-4020-4457-1 e-book P.O. Box 17, 3300 AA Dordrecht, The Netherlands. All Rights Reserved 2006 Springer No part of this work may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording or otherwise, without written permission from the Publisher, with the exception of any material supplied specifically for...
Materials And Methods Eur
We used normal human astrocyte Cambrex Bio Science, Walkersville, MD . NHA were seeded into 12-well plates for quantitative RT-PCR at a density of 2 x 104 well, or 24-well plates for MTT assay at a density of 1 x 104 well in ABM medium Cambrex Bio Science supplemented with 5 FCS, rhEGF and IGF, and cultured in a humidified atmosphere of 5 CO2 in 95 air at 37 C. Stock solutions of PLLA were made in dimethyl sulfoxide DMSO and final concentration of DMSO was 0.1 this concentration did not affect...
Materials And Methods Ihs
Beta- 1,3-1,6 -linked glucan DS- G was received from DAISO CO., LTD. Osaka, Japan . The -glucan was prepared by Daiso Co., Ltd. as follows The glucan was supplied as a soluble microparticle glucan obtained from the culture medium of Aureobasidium pullulans strain 1A1, grown in Czapek medium containing 3 sucrose as the sole carbon source and 0.3 ascorbic acid, sodium salt, under aerobic fermentation conditions for 3-7 days at 27oC. The highly purified -glucan 85-95 in purity was prepared by...
Trace Element Optimization Enhances Performance And Reproducibility Of
Scott J. Jacobia1, Robert W. Kenerson1, Lia D. Tescione1, Dale F. Gruber1, David W. Jayme2, Donald G. Munroe1, and Stephen F. Gorfien1 1Invitrogen Corporation, 3175 Staley Road, Grand Island, New York 14072, USA Department of Biochemistry, Brigham Young University Hawaii, 55-220 Kulanui Street, Laie, Hawaii 96762, USA Abstract Optimizing the quantity and composition of trace elements in the AEM Adenovirus Expression Medium formulation, resulted in an improved version of AEM, which is a...
Results 1
We firstly studied effects of VSV-G on virus titer by changing the amount of the expression vector for the transfection, since the protein has cytotoxic effects on host cells Ory et al., 1996 . We found that 100 to 200 ng of VSV-G expression plasmid DNA per 24 well plate Iwaki gave a good result data not shown . We performed similar experiments on virus vectors and gag pol for 293FT cells expression plasmids to determine the amount of DNA which gave the best results data not shown . In the...
Using Metabolomics To Better Understand Functional Foods
Dayan B. Goodnowe1, Yasuyo Yamazaki1, Bernhard H. J. Juurlink2 1Phenomenome Discoveries Inc. 204-407 Downey Road, Saskatoon, SK, S7N 4L8, Canada 2Department of Anatomy and Cell Biology, College of Medicine, University of Saskatchewan, Saskatoon, SK, S7N 5E5, Canada Abstract Decreasing hypertension and inflammation are important human health objectives. Functional foods are being developed to provide an alternative treatment for mild to moderate symptoms as well as for alternatives to...
PROTEOME ANALYSIS OF NEURITE DIFFERENTIATION OF PHYTOESTROGENTREATED PC12 CELLS
Hiroko Isoda, Mariko Seki, Terence P. N. Talorete, Junkyu Han Graduate School of Life and Environmental Sciences, University of Tsukuba, Japan Abstract A novel isoflavone, 7, 2', 4'-trihydroxyisoflavone-4'-O-P- D-glucopyranoside was isolated from the aerial part of Crotalaria sessiliflora. The isoflavone glucoside was found to enhance the proliferation of the MCF-7 human breast cancer cell line, which possesses estrogen receptors ERs and responds in culture to estrogen. The estrogenic property...
DOWNREGULATION OF a5p1 INTEGRIN EXPRESSION DURING NEURONAL DIFFERENTIATION IN
Dai Muramatsu, Naoko Yoshida, Sohei Hishiyama, Yusei Miyamoto, Tatsuhiro Hisatsune Department of integrated biosciences, University of Tokyo, Bioscience building 402, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan Abstract During neuronal differentiation, dynamic morphological and physiological changes found in neural stem cells imply the involvement of cell adhesion molecules. Integrins, which are major family of cell adhesion molecule play a central role in migration, proliferation and...
Addition of Trace Element Supplement to 293 II SFM
Stock cells were grown in 293 SFM II to a day 4 cell density of approximately 2.5 x 106 vc mL 99 viable and passaged into the four test conditions. Due to the relatively high density of the stock cells all passage 1 test cultures lagged. After two subsequent passages all conditions recovered and achieved day 4 cell densities of approximately 2.5 x 106 vc mL. These cultures were then passaged on day 6 after reaching cell densities in excess of 3.0 x 106 vc mL. The cells in the unsupplemented...
Cell culture on ToshiSubstrata made of adult bovine pancreas
3.2.1 Effect of Section Thickness on Cell Morphology Pancreas-derived TOSHI-substrata cut into 5 or 10 .m-thick sections were prepared and the effect of section thickness on the culture was examined. HIT-T15 cells were seeded on the TOSHI-substrata and microscope observation revealed that they adhered and spontaneously aggregated on both sized sections Fig. 2 no significant difference was observed. This suggests that the TOSHI-substrata can induce cells to aggregate regardless of section...
References 1
Baert FJ, D'Haens GR, Peeters M, Hiele MI, Schaible TF, Shealy D, Geboes K, and Rutgeerts PJ 1999 Tumor necrosis factor alpha antibody infliximab therapy profoundly down-regulates the inflammation in Crohn's ileocolitis. Gastroenterology. 116 1 22-28. Cario E, Becker A, Sturm A, Goebell H, and Dignass AU 1999 Peripheral blood mononuclear cells promote intestinal epithelial restitution in vitro through an pathway. Scand. J. Gastroenterol. 34 1132-1138. Dehouck B, Dehouck MP, Fruchart JC and...
Mesenchymal Cells Metalloproteinases And Adhesion On Microcarriers
C. Mosbeux1, A.R. dos Santos Pedregal1, D. Ribeiro de Sousa1 , Vincianne Hendrick1, N. Joseph, M. Bensellam1, D. Blankaert1, T. Marique1, C. Alloin1, D. Parent2, C. Liesnard2, J.P. Van Vooren2, S. Lowagie1 and J. Werenne1 1Laboratory of Animal Cell Biotechnology. Dept. of Bioengineering, Faculty of Sciences, Universit Libre de Bruxelles, Belgium and 2Erasme Hospital,, Brussels, Belgium. Abstract The purpose of this work was to approach the development of a safe and validable process to grow the...